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报告题目 |
Bioimaging at the nanoscale: Single-molecule and super-resolution fluorescence microscopy |
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报告时间 |
2012年1月4日上午10：00 |
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报告地点 |
合肥微尺度物质科学国家实验室，18层大楼西三报告厅 |
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报告摘要 |
Optical microscopy is an essential tool in biological research. However, the spatial resolution of optical microscopy, classically limited by diffraction to several hundred nanometers, is substantially larger than typical molecular length scales in cells, leaving many biological structures unresolvable. We recently developed a new form of super-resolution light microscopy, stochastic optical reconstruction microscopy (STORM), that surpasses the diffraction limit. STORM uses single-molecule imaging and photoswitchable fluorescent probes to temporally separate the spatially overlapping images of individual molecules. This approach allows the localization of fluorescent probes with nanometer precision and the construction of sub-diffraction-limit images. Using this method, we have achieved multicolor and three-dimensional (3D) imaging of living cells with nanometer-scale resolution. In this talk, I will discuss the general concept, recent technical advances and biological applications of STORM. |
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报告人 |
Prof. Xiaowei Zhuang, Department of Chemistry and Chemical Biology, Department of Physics, Howard Hughes Medical Institute, Harvard University, USA |
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报告人简介 庄小威教授，国际著名生物物理学家，美国霍华德休斯医学研究所研究员，哈佛大学化学和化学生物系、物理系双聘教授、中国科大“大师讲席”教授。15岁时庄小威考入中国科学技术大学少年班，25岁获得美国加州大学伯克利分校物理学博士学位，之后师从著名生物物理学家、1997诺贝尔物理学奖获得者朱棣文教授，在斯坦福大学做生物物理学博士后研究。她在单分子动力学、核酸与蛋白的相互作用、基因表达机制、细胞核病毒的相互作用等领域做出了杰出的贡献。近年来发明了突破光学衍射极限的STORM荧光成像技术，使得光学显微镜分辨能力接近纳米尺度，极大地推动了亚细胞微观结构的研究。她连续多年在Nature，Science，Cell，Nature Methods，Nature Biology，Neuron，PNAS等重要学术期刊上发表多篇论文，并获得MacArthur Fellowship，HHMI collaborative Innovation Award，TR Worlds Top 100 Young Innovators Award，Max Delbruck Prize in Biological Physics，Raymond & Beverly Sackler International Prize in Biophysics等著名奖项和荣誉。最近她又荣获2011年度赛克勒国际生物物理学奖。 | |
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主办单位：化学与材料学院，微尺度国家实验室，研究生院 | |